It's more complicated than just barrel length. Transfer port size has an effect ( to big or to small), dwell time, and the flow rate through the valve. Flow rate through the valve is not always optimal, it can be better or worse at different pressures.
Yes, the short barrel has a limiting factor on power output. The same as a longer barrel does but more so. This is because you want to tune the valve to be the most efficient at any given regulator set point. You want to tune so that the valve is closed back just before the ammo exits the muzzle to prevent wasted air from exiting behind the ammo. This means the valve in a shorter barrel has to close a little faster than in a longer barrel. So, not as much time for the shorter barrel to build as much pressure compared to a longer barrel.When tuning I would find the max velocity for the current set point and then back off on that max velocity by 5% by adjusting the hammer energy. If you don't like the end result of this velocity, then adjust the reg and start over. When doing this you'll find the gun to be in its sweet spot and tune just below the knee of the bell curve. You should notice the highest shot count and the quietest report as well. You may also notice an increase in accuracy if the prior tune was letting out enough air behind the ammo before the valve was closing. This can cause the ammo to skew when exiting the muzzle.
A spacer on the valve spring probably won't make the valve close faster, because the closing pressure on the poppet is probably greater than the spring rate, even spaced. A bigger poppet diameter would close faster, all things being equal.